Lease/Rent

Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source

Listing #:LS-006550
Brand:Agilent
Model:6465B Ultivo
Availability:In Stock
Condition:Refurbished
Warranty:90 Days
Onsite Installation:Available upon request
$96,700.00
In Stock
Make an Offer

Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source — a compact, stackable LC/MS/MS that eliminates the separate mass spec footprint by integrating directly into the HPLC stack. Equipped with standard Electrospray Ionization (ESI), Vortex Collision Cell, Cyclone Ion Guide, and VacShield technology. Refurbished and tested by Alliant Scientific.

Listing last updated: September 2026

Description

Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source Overview

Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source represents a transformative approach to triple quadrupole mass spectrometry, delivering the fit-for-purpose, rugged LC/MS analysis expected from larger comparable systems at a fraction of their size. It’s a compact, stackable design that eliminates the separate MS footprint entirely by incorporating the mass spectrometer directly into the HPLC stack, maximizing lab bench space without compromising performance.

The system performs MS/MS using three sets of parallel rods (quadrupole, hexapole, quadrupole) — the first quadrupole selects precursor ions, which are fragmented in the hexapole into product ions and separated by the second quadrupole. Using Dynamic MRM, the Ultivo can monitor up to 4,000 MRM transitions in a single run.

This configuration is equipped with a standard Electrospray Ionization (ESI) source, a proven, versatile ionization technique well suited to a broad range of polar and moderately polar compounds across standard-flow LC-MS/MS workflows. Innovations built into the Ultivo include the Vortex Collision Cell for improved ion fragmentation and transmission (enabling more MRMs per second with greater accuracy and confidence), the Cyclone Ion Guide for smoother ion transmission between vacuum zones and better signal quality, and VacShield technology, which allows capillary changes without venting the system — reducing downtime and improving day-to-day lab efficiency. The system is compatible with 1260 Infinity II, 1260 Infinity Prime, or 1290 Infinity II LC stacks.

The Ultivo supports 21 CFR Part 11 compliance, making it well suited for regulated environments, and is used across food safety, environmental, drug discovery, toxicology, forensics, bioanalysis, protein quantitation, clinical, academic, pharmaceutical, and veterinary drug applications.

This Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source is offered fully refurbished and tested, in working condition, by Alliant Scientific. Find the instrument’s tune report, verifying performance prior to sale, under Downloads.

Includes:

  • Agilent 6465B Ultivo Triple Quad LC/MS with ESI Source System
    • Electrospray Ionization (ESI) Source
    • Roughing Pump
  • Data System
  • 90-Day Warranty

Optional:

  • Agilent 1260/1290 Infinity II LC Front End

Disclaimer: All brand names and trademarks are the property of their respective owners and are used for identification only. We are not an authorized dealer. Software, if included, is not transferable or upgradable.

Browse More LC/MS Systems

Looking for higher desolvation efficiency and sensitivity? See our Agilent 6465B Ultivo with Agilent Jet Stream (AJS) Source configuration, or browse our full LC-MS inventory.

Technical Specifications

Manufacturer Agilent Technologies
Model 6465B Ultivo (G6465B)
Type Compact, Stackable Triple Quadrupole LC/MS
Ion Source Electrospray Ionization (ESI)
MRM Capacity Up to 4,000 transitions (Dynamic MRM)
Collision Cell Vortex Collision Cell — improved fragmentation and MRM throughput
Ion Guide Cyclone Ion Guide — smoother ion transmission, improved signal quality
Maintenance Feature VacShield — capillary changes without venting the system
Compliance 21 CFR Part 11 compatible
LC Compatibility 1260 Infinity II, 1260 Infinity Prime, 1290 Infinity II
Dimensions (W x D x H) 32 x 88 x 39.5 cm
Country of Origin Singapore
Software Agilent MassHunter
Condition Refurbished and tested
Warranty 90 days

Additional information

Availability

Brand

Condition

Model

Onsite Installation

Warranty

Key Features

  • Compact, Stackable Design
    Eliminates the separate MS footprint by integrating into the HPLC stack, saving bench space.
  • Standard ESI Source
    Proven, versatile electrospray ionization suited to a broad range of polar and moderately polar compounds.
  • Vortex Collision Cell
    Improves ion fragmentation and transmission, enabling more MRMs per second.
  • Cyclone Ion Guide
    Smoother ion transmission between vacuum zones with improved signal quality.
  • VacShield Technology
    Change capillaries without venting the system, reducing maintenance downtime.
  • High MRM Capacity
    Monitors up to 4,000 MRM transitions using Dynamic MRM.

Applications

  • Food Safety Testing
    Pesticide and contaminant screening in complex food matrices.
  • Environmental Studies
    Trace organic compound analysis in environmental samples.
  • Toxicology & Forensics
    Compound identification and quantitation for toxicology and forensic casework.
  • Clinical & Pharmaceutical
    Protein quantitation and pharmaceutical compound analysis.

FAQ (Frequently Asked Questions)

What’s the difference between this ESI version and the Agilent Jet Stream (AJS) version?

Both use the same Ultivo G6465B platform, but AJS adds a heated sheath gas design that dramatically increases the number of ions entering the mass spectrometer, improving desolvation efficiency and sensitivity, particularly at higher flow rates. Standard ESI remains a proven, versatile choice for a wide range of standard-flow LC-MS/MS applications.

How many MRM transitions can this system monitor?

Up to 4,000 MRM transitions using Dynamic MRM.

What is VacShield technology?

VacShield allows capillary changes without venting the system, reducing downtime and improving lab efficiency.

What LC systems is this compatible with?

Agilent 1260 Infinity II, 1260 Infinity Prime, or 1290 Infinity II LC stacks.

Does this unit include a warranty?

Yes — this unit ships with a 90-day warranty.