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Agilent 6495B Triple Quad LC/MS
$138,500.00
In Stock
Compare Related Systems
Looking at other Triple Quad options? The Agilent 6490 offers similar iFunnel sensitivity at a lower mass range (m/z 5–1,400), while the Agilent 6460 and Agilent 6430 are solid entry points for labs that don’t need the 6495B’s extended peptide-range capability.
Description
Quick Answer
Agilent 6495B is a Triple Quad LC/MS system built on Agilent’s iFunnel platform, delivering ultra-trace sensitivity with an extended m/z 5–3,000 mass range for peptide quantitation and demanding multi-residue methods. This refurbished unit includes the Agilent Jet Stream source and is fully tested by Alliant Scientific.
Listing last updated: August 2026
Agilent 6495B Triple Quad LC/MS Overview
This system delivers ultra-trace sensitivity down to sub-picogram/attogram levels, and its extended m/z 5–3,000 range makes it especially well suited for large peptide fragment work, not just standard small-molecule quantitation.
The Agilent 6495B Triple Quad LC/MS System is a high-performance liquid chromatography–tandem mass spectrometry platform built for laboratories that need maximum sensitivity, wide dynamic range, and reliable quantitation across complex sample types. At its core is Agilent’s iFunnel ion optics design, which improves ion capture and transmission efficiency into the mass analyzer — allowing detection of compounds at extremely low concentrations that are difficult to resolve on conventional LC-MS/MS systems.
The 6495B extends the mass range to m/z 3,000, which makes it a strong fit for large peptide fragment detection alongside standard small-molecule quantitation — a meaningful step up from earlier 6495-series units for labs doing peptide or protein-fragment work. It’s optimized for multiple reaction monitoring (MRM) workflows, with fast acquisition speeds that support large-scale multi-residue and multi-transition methods without sacrificing dwell time or reproducibility.
Paired with the Agilent Jet Stream (AJS) source, the system maintains strong signal stability across demanding matrices — biological fluids, environmental extracts, food and spice matrices — and is fully compatible with fast UHPLC methods, capturing narrow peak widths while preserving data density for accurate quantitation. Rapid polarity switching allows simultaneous positive/negative ionization workflows without extending run time.
Agilent 6495B Triple Quad LC/MS is offered fully refurbished and tested, in working condition by Alliant Scientific.
Includes:
- Agilent 6495B Triple Quad LC/MS
- Agilent Jet Stream (AJS) Source
- Vacuum Pump(s)
- Data System
- 90-Day Warranty
Optional:
- Agilent UHPLC Front End Systems (1260 or 1290 Infinity Series)
- Premium Refurbishment with Extended Warranty
Disclaimer: All brand names and trademarks are the property of their respective owners and are used for identification only. We are not an authorized dealer. Software, if included, is not transferable or upgradable.
Technical Specifications
| General | |
|---|---|
| Instrument Type | Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) |
| Model | Agilent 6495B (G6495B) |
| Mass Analyzer | Triple Quadrupole (QQQ) |
| Ionization Source | Agilent Jet Stream (AJS) ESI, optional multi-mode sources |
| Application Range | Ultra-trace quantitative analysis, peptide/protein fragment detection |
| Mass Spectrometer Performance | |
| Mass Range | m/z 5 – 3,000 |
| Sensitivity | Sub-picogram to attogram-level detection (compound dependent) |
| Mass Accuracy | 0.1 Da (m/z 5–1,000) to 0.3 Da (m/z 2,000–3,000) |
| Mass Stability | <0.1 Da over 24 hours |
| Dynamic Range | Up to ~10⁶ |
| MRM / Quantitative Performance | |
| Acquisition Modes | MRM, Dynamic MRM, SIM, Full Scan, Product Ion Scan |
| Detection Technology | iFunnel with high energy conversion dynode |
| Quantitation Capability | Ultra-trace multi-analyte analysis with high selectivity |
| Ion Optics & Sensitivity | |
| Ion Transmission | Enhanced iFunnel ion focusing for improved signal intensity |
| Desolvation Technology | Thermally assisted ion focusing (Jet Stream design) |
| LC Compatibility | |
| Supported Systems | Agilent 1200 / 1260 / 1290 HPLC & UHPLC series |
| UHPLC Compatibility | Optimized for fast separations and narrow peak widths |
| Software & Control | |
| Software | Agilent MassHunter Workstation |
| Functionality | Method development, data acquisition, quantitation, reporting |
Additional information
| Availability | |
|---|---|
| Condition | |
| Model | |
| Onsite Installation | |
| Warranty | |
| Brand |
Key Features
- Ultra-Sensitive Detection Performance
Sub-picogram to attogram-level sensitivity via third-generation iFunnel technology, suited for ultra-trace analysis. - Extended Mass Range for Peptide Work
m/z 5–3,000 range accommodates large peptide fragment detection alongside standard small-molecule quantitation. - Advanced Ion Transmission Design
iFunnel ion optics significantly improve signal strength and transmission efficiency into the analyzer. - High-Speed Acquisition for UHPLC
Captures narrow chromatographic peaks with precision, supporting fast LC separations without data loss. - Rapid Polarity Switching
Alternates between ion modes efficiently, reducing total method run time. - Integrated Software Workflow
Controlled through Agilent MassHunter for streamlined method development, acquisition, and reporting.
Applications
- Peptide & Protein Fragment Quantitation
Extended mass range supports large peptide fragment detection for proteomics workflows. - Pharmaceutical Analysis
Quantitative drug analysis, metabolite profiling, and regulatory testing workflows. - Food Safety Testing
Simultaneous screening of mycotoxins, illegal dyes, and pesticide residues in complex matrices like spices. - Environmental Monitoring
Detection of trace contaminants and pollutants in water, soil, and air samples. - Clinical & Biomarker Research
Accurate quantitation of biological markers in complex biological matrices.
FAQ (Frequently Asked Questions)
What is the Agilent 6495B best used for?
It’s designed for ultra-trace quantitative analysis, including peptide and protein fragment detection thanks to its extended m/z 5–3,000 mass range, along with standard small-molecule multi-residue methods.
What is the difference between the 6495, 6495B, 6495C, and 6495D?
The original 6495 introduced iFunnel and Jet Stream technology. The 6495B extended the mass range to m/z 3,000 for large peptide fragment work. The 6495C added a third-generation all-metal iFunnel design and VacShield for easier maintenance, pushing sensitivity into the ppt range. The 6495D (2023) adds AI-based SWARM tuning and an early maintenance feedback dashboard for high-throughput, heavy-matrix labs.
What mass range does the 6495B support?
m/z 5 to 3,000 — wide enough for both small-molecule quantitation and large peptide fragment detection.
Is the 6495B compatible with UHPLC systems?
Yes, it’s fully compatible with modern UHPLC setups and performs well with fast, narrow chromatographic peaks.
What ion source does the 6495B use?
It uses the Agilent Jet Stream (AJS) source for enhanced ionization efficiency and sensitivity.
Does this unit include a warranty?
Yes — 90-day warranty.
