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Description
Agilent 1200 HPLC GPC System with RID Detector and Iso Pump
Optimized Gel Permeation Chromatography (GPC/SEC) Platform for Polymer Analysis
Agilent 1200 HPLC GPC System with RID Detector and Iso Pump is a purpose-built solution for gel permeation chromatography (GPC) and size-exclusion chromatography (SEC), enabling precise characterization of polymer molecular weight distribution, size, and composition. Designed around a stable isocratic flow architecture, this system ensures consistent mobile phase conditions—an essential requirement for accurate refractive index detection. This stability directly translates into reliable retention times, reproducible separations, and dependable quantitative results, especially in polymer analysis workflows.
The system delivers excellent baseline stability and low noise performance, which are critical for detecting subtle differences in polymer concentration and molecular size. Its integrated thermal control minimizes fluctuations in temperature that could otherwise affect solvent viscosity and refractive index, ensuring consistent detector response and improved data accuracy.
Optimized for GPC applications, this platform allows laboratories to determine key polymer parameters such as molecular weight averages (Mw, Mn) and polydispersity index (PDI) when used with appropriate calibration standards. Its precise sample introduction and automated operation make it well-suited for both research and routine quality control environments. With its universal detection capability, the system can analyze a wide range of materials that lack UV absorbance, including synthetic polymers, resins, and natural macromolecules. This makes it a valuable tool across industries such as materials science, chemical manufacturing, plastics, and academic research.
This refurbished Agilent 1200 HPLC GPC System has been fully tested and verified to be in proper working condition by Alliant Scientific.
Includes:
- Agilent 1200 HPLC GPC System
- Solvent Tray
- G1322A Degasser
- G1310A Isocratic Pump
- G1329B Autosampler
- G1316A TCC
- G1362A RID
- Data System
- 90-Day Warranty Coverage
Optional:
- Premium refurbishment available with extended warranty options
Disclaimer:Â All brand names and trademarks are the property of their respective owners and are used for identification only. We are not an authorized dealer. Software, if included, is not transferable or upgradable.
Technical Specifications
| System Type | Isocratic HPLC system configured for GPC/SEC polymer analysis |
| Separation Technique | Gel Permeation Chromatography (GPC) / Size-Exclusion Chromatography (SEC) |
| Flow Rate Range | 0.001 – 10 mL/min with high precision and reproducibility |
| Operating Pressure | Up to ~400 bar |
| Injection Volume Range | ~0.1 – 100 µL |
| Temperature Control | Thermostatted column environment (ambient to ~80°C) |
| Detection Method | Refractive Index Detection (RID) for universal compound detection |
| Detection Capability | Measures concentration of polymers independent of UV absorbance |
| Baseline Performance | Low noise and low drift under stable isocratic conditions |
| Automation | Sequence-based batch analysis for high-throughput workflows |
| Power Requirements | 100 – 240 V, 50/60 Hz |
Additional information
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Key Features
- Optimized for GPC/SEC Analysis: Designed for accurate separation of polymers based on molecular size and hydrodynamic volume
- Reliable Molecular Weight Determination: Enables calculation of Mw, Mn, and polydispersity index (PDI) when used with calibration standards
- Stable Isocratic Operation: Maintains constant solvent composition essential for reproducible refractive index detection
- Universal Detection Capability: Detects polymers and compounds without UV absorbance using refractive index detection
- Low Baseline Drift and Noise: Provides stable signal performance for accurate quantitation of polymer samples
- Temperature-Controlled Analysis: Reduces variation in solvent viscosity and refractive index for consistent results
- High Reproducibility: Ensures consistent retention times and peak profiles across repeated runs
- Efficient Batch Processing: Supports automated workflows for routine polymer and QC analysis
- Proven Agilent Platform: Built on the reliable 1200 Series for long-term performance in analytical labs
Applications
- Polymer Molecular Weight Analysis: Determines molecular weight distribution (Mw, Mn) and polydispersity index (PDI)
- Size-Exclusion Chromatography (SEC): Separates macromolecules based on hydrodynamic volume without interaction effects
- Plastics and Polymer Characterization: Analyzes synthetic polymers, resins, and industrial materials
- Biopolymer Analysis: Characterizes proteins, polysaccharides, and natural macromolecules
- Chemical and Materials R&D: Supports polymer development, synthesis monitoring, and formulation work
- Quality Control Laboratories: Verifies batch consistency in polymer manufacturing processes
Frequently Asked Questions (FAQ)
- What is this system used for?
This system is used for gel permeation chromatography (GPC/SEC) to analyze polymer size and molecular weight distribution. - Why use refractive index detection in GPC?
Most polymers do not absorb UV light, so refractive index detection provides universal measurement based on concentration. - What polymer data can this system generate?
It can provide molecular weight averages (Mw, Mn) and polydispersity index (PDI) when calibrated with standards. - Why is isocratic flow important?
Constant solvent composition ensures stable refractive index measurements and prevents baseline drift. - How does temperature impact performance?
Temperature affects solvent viscosity and refractive index, so stable control is critical for reproducibility. - What types of samples can be analyzed?
Synthetic polymers, plastics, resins, and natural macromolecules can all be analyzed using this system. - Is this system suitable for routine lab use?
Yes, it supports both research applications and high-throughput quality control environments.
