Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader
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Description
Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader
The Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is a multi-mode microplate detection system designed for absorbance, fluorescence intensity, and luminescence measurements in life science research laboratories. The SpectraMax M5e combines monochromator-based wavelength selection with flexible detection capabilities, allowing researchers to perform multiple assay types on a single platform without requiring filter changes.
The system utilizes tunable monochromators for both excitation and emission wavelength selection, providing flexibility when developing or optimizing assays. Its broad wavelength range supports DNA and RNA quantification, protein analysis, ELISA testing, cell viability assays, enzyme kinetics, reporter gene analysis, and fluorescence-based applications. The monochromator design allows users to select virtually any wavelength within the instrument’s operating range while eliminating the need for dedicated optical filters.
The SpectraMax M5e is commonly used in molecular biology, cell biology, biochemistry, drug discovery, pharmaceutical research, biotechnology laboratories, and academic research environments. The instrument supports both endpoint and kinetic measurements, enabling rapid data acquisition across a wide variety of microplate-based assays.
This Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is offered fully tested, in working condition by Alliant Scientific.
Includes:
- Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader
- Data System
Disclaimer:Â All brand names and trademarks are the property of their respective owners and are used for identification only. We are not an authorized dealer. Software, if included, is not transferable or upgradable.
Technical Specifications
| Manufacturer | Molecular Devices |
| Model | SpectraMax M5e |
| Instrument Type | Multi-Mode Microplate Reader |
| Detection Modes | Absorbance, Fluorescence Intensity, Luminescence |
| Absorbance Wavelength Range | 200–1000 nm |
| Fluorescence Excitation Range | 250–850 nm |
| Fluorescence Emission Range | 250–850 nm |
| Luminescence Detection | Glow and Flash Luminescence |
| Optical System | Dual Monochromator Design |
| Plate Formats | 6-, 12-, 24-, 48-, 96-, and 384-Well Plates |
| Read Modes | Endpoint, Kinetic, Spectral Scanning, Well Area Scanning |
| Wavelength Selection | Monochromator Based |
| Temperature Control | Ambient +4°C to 45°C |
| Shaking Modes | Linear, Orbital, Double Orbital |
| Detector Type | Photomultiplier Tube (PMT) |
| Dimensions (W × D × H) | Approx. 22 × 18 × 9 in (56 × 46 × 23 cm) |
| Weight | Approx. 50 lb (23 kg) |
| Power Requirements | 100–240 VAC, 50/60 Hz |
Additional information
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Key Features
- Multi-Mode Detection
Performs absorbance, fluorescence intensity, and luminescence assays on a single instrument platform. - Monochromator-Based Optics
Allows flexible wavelength selection without the cost and limitations associated with optical filters. - Broad Spectral Range
Supports diverse life science applications across ultraviolet, visible, and fluorescence wavelengths. - Flexible Plate Compatibility
Accommodates multiple plate formats for research, screening, and assay development workflows. - Kinetic and Endpoint Measurements
Supports enzyme kinetics, cell-based assays, and quantitative endpoint analysis. - Integrated Incubation and Shaking
Provides temperature control and automated plate shaking for assay optimization.
Applications
- ELISA and immunoassays
- DNA and RNA quantification
- Protein concentration assays
- Cell viability and proliferation studies
- Reporter gene assays
- Enzyme kinetics measurements
- Fluorescence-based cellular assays
- Drug discovery and screening
- Molecular biology research
- Biochemical and pharmaceutical analysis
FAQ
- What is the Molecular Devices SpectraMax M5e Microplate Reader used for?
The Molecular Devices SpectraMax M5e Microplate Reader is used for absorbance, fluorescence, and luminescence assays in life science and pharmaceutical research laboratories. - What detection modes are available on the SpectraMax M5e?
The SpectraMax M5e supports absorbance, fluorescence intensity, and luminescence measurements. - Does the Molecular Devices SpectraMax M5e use filters?
No. The SpectraMax M5e utilizes monochromator-based wavelength selection, providing flexibility without changing optical filters. - What microplate formats are compatible with the SpectraMax M5e?
The instrument supports 6-, 12-, 24-, 48-, 96-, and 384-well microplates. - Can the Molecular Devices SpectraMax M5e perform kinetic measurements?
Yes. The system supports both endpoint and kinetic reading modes for enzyme assays and time-course studies. - Is the Molecular Devices SpectraMax M5e suitable for fluorescence assays?
Yes. The broad excitation and emission wavelength ranges support a wide variety of fluorescence-based applications.









